hrecs cat. Search Results


90
Lonza primary human renal tubular epithelial (hre) cells
Primary Human Renal Tubular Epithelial (Hre) Cells, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary human renal tubular epithelial (hre) cells/product/Lonza
Average 90 stars, based on 1 article reviews
primary human renal tubular epithelial (hre) cells - by Bioz Stars, 2026-05
90/100 stars
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90
ScienCell hrecs cat. #6530
Effects of Cx43 knockdown on HG-induced angiogenesis (A) Cx43 knockdown in <t>hRECs</t> was achieved through the transfection of the vector containing small hairpin RNA targeting Cx43 (sh-Cx43#1/2/3); Cx43 knockdown was confirmed using Immunoblotting. hRECs were subsequently transfected with sh-Cx43#2 (with better knocking down <t>efficiency),</t> <t>cultured</t> in normal or 60 mmol/L HG medium, and examined for tubule formation on Matrigel (B) ; ROS release using flow cytometry (C) ; the content of TNF-α, IL-1β, VEGFA, and ICAM-1 in culture medium using ELISA kits (D) . n = 3, *p < 0.05, **p < 0.01 compared with normal+sh-NC group; ## p < 0.01 compared with HG+sh-NC group.
Hrecs Cat. #6530, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hrecs cat. #6530/product/ScienCell
Average 90 stars, based on 1 article reviews
hrecs cat. #6530 - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier

Image Search Results


Effects of Cx43 knockdown on HG-induced angiogenesis (A) Cx43 knockdown in hRECs was achieved through the transfection of the vector containing small hairpin RNA targeting Cx43 (sh-Cx43#1/2/3); Cx43 knockdown was confirmed using Immunoblotting. hRECs were subsequently transfected with sh-Cx43#2 (with better knocking down efficiency), cultured in normal or 60 mmol/L HG medium, and examined for tubule formation on Matrigel (B) ; ROS release using flow cytometry (C) ; the content of TNF-α, IL-1β, VEGFA, and ICAM-1 in culture medium using ELISA kits (D) . n = 3, *p < 0.05, **p < 0.01 compared with normal+sh-NC group; ## p < 0.01 compared with HG+sh-NC group.

Journal: Frontiers in Endocrinology

Article Title: Connexin 43 (Cx43) regulates high-glucose-induced retinal endothelial cell angiogenesis and retinal neovascularization

doi: 10.3389/fendo.2022.909207

Figure Lengend Snippet: Effects of Cx43 knockdown on HG-induced angiogenesis (A) Cx43 knockdown in hRECs was achieved through the transfection of the vector containing small hairpin RNA targeting Cx43 (sh-Cx43#1/2/3); Cx43 knockdown was confirmed using Immunoblotting. hRECs were subsequently transfected with sh-Cx43#2 (with better knocking down efficiency), cultured in normal or 60 mmol/L HG medium, and examined for tubule formation on Matrigel (B) ; ROS release using flow cytometry (C) ; the content of TNF-α, IL-1β, VEGFA, and ICAM-1 in culture medium using ELISA kits (D) . n = 3, *p < 0.05, **p < 0.01 compared with normal+sh-NC group; ## p < 0.01 compared with HG+sh-NC group.

Article Snippet: hRECs (Cat. #6530, ScienCell, Carlsbad, CA, USA) were cultured (37°C, 5% CO 2 ) in Endothelial Cell Medium (ECM, Cat. #1001, ScienCell) containing 5% fetal bovine serum (FBS; Cat. #0025, ScienCell). hRECs were allocated into four groups: normal, low-HG (culture medium containing 20 mmol/L glucose), middle-HG (culture medium containing 60 mmol/L glucose), and high-HG (culture medium containing 120 mmol/L glucose).

Techniques: Knockdown, Transfection, Plasmid Preparation, Western Blot, Cell Culture, Flow Cytometry, Enzyme-linked Immunosorbent Assay

Effects of Cx43 overexpression on HG-induced angiogenesis (A) Cx43 overexpression in hRECs was achieved through transfection of the vector containing Cx43 gene coding sequence (Cx43 OE); Cx43 overexpression was confirmed using Immunoblotting. hRECs were subsequently transfected with Cx43 OE, cultured in normal or 60 mmol/L HG medium, and examined for tubule formation on Matrigel (B) ; ROS release using flow cytometry (C) ; the content of TNF-α, IL-1β, VEGFA, and ICAM-1 in culture medium using ELISA kits (D) . n = 3, **p < 0.01 compared with normal+vector group; ## p < 0.01 compared with HG+vector group.

Journal: Frontiers in Endocrinology

Article Title: Connexin 43 (Cx43) regulates high-glucose-induced retinal endothelial cell angiogenesis and retinal neovascularization

doi: 10.3389/fendo.2022.909207

Figure Lengend Snippet: Effects of Cx43 overexpression on HG-induced angiogenesis (A) Cx43 overexpression in hRECs was achieved through transfection of the vector containing Cx43 gene coding sequence (Cx43 OE); Cx43 overexpression was confirmed using Immunoblotting. hRECs were subsequently transfected with Cx43 OE, cultured in normal or 60 mmol/L HG medium, and examined for tubule formation on Matrigel (B) ; ROS release using flow cytometry (C) ; the content of TNF-α, IL-1β, VEGFA, and ICAM-1 in culture medium using ELISA kits (D) . n = 3, **p < 0.01 compared with normal+vector group; ## p < 0.01 compared with HG+vector group.

Article Snippet: hRECs (Cat. #6530, ScienCell, Carlsbad, CA, USA) were cultured (37°C, 5% CO 2 ) in Endothelial Cell Medium (ECM, Cat. #1001, ScienCell) containing 5% fetal bovine serum (FBS; Cat. #0025, ScienCell). hRECs were allocated into four groups: normal, low-HG (culture medium containing 20 mmol/L glucose), middle-HG (culture medium containing 60 mmol/L glucose), and high-HG (culture medium containing 120 mmol/L glucose).

Techniques: Over Expression, Transfection, Plasmid Preparation, Sequencing, Western Blot, Cell Culture, Flow Cytometry, Enzyme-linked Immunosorbent Assay